Amplicon sequencing of soil in Tricholoma matsutake habitat

This study performed amplicon sequencing of DNA extracted from soil samples collected in natural habitats of Tricholoma matsutake. The 16S rRNA gene (V4 region, using primers 515F-806R) and the ITS region (for fungi) were amplified and sequenced on the Illumina MiSeq platform. The objective was to compare microbial community composition between T. matsutake-dominant areas and adjacent soils with minimal T. matsutake presence, to explore potential microbial associations relevant to ectomycorrhizal symbiosis. This project was originally performed by Su-Min Kim ([e-mail])