Effects of atropine during pretreatment with 3 µM xanomeline or following washout on [3H]NMS saturation binding parameters and activation of PI hydrolysis by carbachol in CHO hM1 cells.

Cells were pretreated for 1 h with xanomeline and/or atropine followed by extensive washing and waiting for 23 h in the absence or presence of atropine. Parameters derived from nonlinear regression analysis of data shown in Fig. 9 are presented as mean ± S.E.M. of three experiments performed in triplicate. All other details as for Tables 2 and 3.aExpressed as percentage of the maximal response to carbachol in untreated cells (25000±2200 dpm).bControl, naïve cells were incubated with radioligand in binding assays, or carbachol in functional assays.*ANOVA followed by Tukey’s post-test detected a significant difference (p