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Expression data of HepG2 cells under siRNA knock down of 13 liver enriched transcription factors (CBX81). unidentified

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https://www.ncbi.nlm.nih.gov/bioproject/PRJDB20058
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资源简介:
To predict the target genes of liver enriched transcription factors in HepG2, we analyzed gene expression after siRNA knock down of each 13 liver enriched transcription factors compared with negative control siRNA (control) using Illumina Sentrix Human-6 Expression BeadChip. For each siRNA knock down, we hybridized four samples, four of biological repllicate. We analyzed data with two steps. First step was normalization of each samples by Quantile Normalization, and after first step we used the method of B statistic for ranking genes. In the analysis of B statistic, we used all of four samples compared with negative control. We analyzed these data set by limma program of bioconductor package in R. See "http://bioconductor.org/packages/2.1/bioc/vignettes/limmaGUI/inst/doc/LinModIntro.pdf" for a discussion of B statistic.
创建时间:
2025-01-20
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