Supplemental Material for Razzaq, et al., 2021

Supplemental figures, tables, and files for Genetics manuscript "The SAGA and NuA4 component Tra1 regulates Candida albicans drug resistance and pathogenesis - Supplemental files"
Figure S1. Regulatory associations of Sfp1 with the tra1Q3 differentially
expressed genes in S. cerevisiae. Sfp1 associations with
differentially expressed genes in the tra1Q3
strain assessed using YEASTRACT+. The experimental evidence underlying each regulatory association (solid
lines for DNA-binding evidence; dashed lines for expression evidence), as well
as the sign of the interaction—positive (green), negative (red), positive and
negative (brown), or undefined (black) are displayed.

Figure S2. Characterization of TRA1 mutant strains. (a) tra1∆/∆ mutants were validated by PCR. Two PCRs (left panel) confirmed the
absence of the TRA1 open reading
frame (ORF) via primers located inside the ORF, and up- or down-stream of the
ORF. Two additional PCRs (right panel) confirmed the integration of the
knockout cassette at the TRA1 locus
(left side), as well as integration of the CRISPR-Cas9 plasmid in the C. albicans genome (right side). For all
PCRs, control strains were added that contain the TRA1 ORF, which include fRS1 (SC5314 wild-type C. albicans), and fRS302 (fRS1 containing a non-targeting Cas9
plasmid integrated in the genome), as well as a no DNA template control. tra1∆/∆ mutant strains ‘1’ and ‘2’ were selected for further phenotypic
characterization. (b) The TRA1 deletion strain is severely impaired in growth. Deletion
strains and corresponding wild-type strains were grown up overnight in liquid
YPD media for 15.5 hours at 37˚C. (c) TRA1 deletion results in severely
impaired growth on solid medium. C.
albicans strains were serially diluted (1:10 dilutions) and spotted onto
solid YPD agar media. (d) tra1Q3 mutant strains are not
defective in overall growth rate. Kinetic growth curves between C. albicans wild-type strain and tra1Q3 mutant strains
indicate no differences in overall growth dynamics over time.

Figure S3. Limited overlap between the Tra1- and
Gcn5-regulated genes in C. albicans.
(a) Volcano plot showing the distribution of the
previously identified 336 differentially expressed genes in gcn5Δ (from (Shivarathri et al. 2019)) within the tra1Q3 dataset. Genes significantly (log2
fold change tra1Q3/TRA1 > 1 and P < 0.05) altered in expression are highlighted in blue
(downregulated) and red (upregulated). (b)
Differentially regulated Gcn5 targets in tra1Q3 dataset are listed in the table with both the log2
fold change within the original gcn5Δ and the tra1Q3 datasets.