Effect of Flt3L and DNGR-1 on the biology of tumor-infiltrating type 1 dendritic cells

DNGR-1 is a dead cell-sensing receptor specifically expressed in type 1 conventional dendritic cells (cDC1s), but whether it plays a role in antitumor immunity remains unexplored. In our work we have explored the transcriptional profile of tumor-infiltrating cDC1s competent or deficient in DNGR-1, Transcriptomic analysis comparing tumor-infiltrating cDC1s from WT and DNGR-1-deficient mice (Clec9agfp/gfp) hydrodynamically injected with a Flt3L-producing plasmid (FL) or an empty plasmid (control). Three replicates of each of the four conditions, where each replicate was generated from tumors of a pool of 2-3 mice.