Genome-wide analysis of gene expression patterns in mir-122 knockout mice livers

To investigate the physiology roles of mir-122 in liver, we performed expression profiling of mir-122 knockout mice and the control B6/129 mice. Total RNA was extracted from four mir-122 knockout mice and three control mice of 2 months of age. Gene expression was quantified by robust multi-array analysis (RMA) using the Genomic Suite software from Partek.为探究mir-122（mir-122）在肝脏中的生理功能，我们对mir-122基因敲除小鼠与对照B6/129小鼠开展了表达谱分析。我们从4只2月龄的mir-122基因敲除小鼠和3只同年龄段对照小鼠中提取总RNA，并采用Partek公司的Genomic Suite软件，通过稳健多阵列分析（robust multi-array analysis，RMA）对基因表达水平进行定量检测。