IKZF1 in BCR-ABL1 driven pre-B cells. Mus musculus

In order to investigate the function of IKZF1 in ALL, we isolated bone marrow cells from C57Bl6 mice and transformed them with BCR-ABL1. In a second transduction the BCR-ABL1 driven pre-B cells were transformed either with IKZF1-GFP or empty vector control (GFP) and subjected to gene expression analysis. Overall design: Three days post transduction, total RNA of GFP positive sorted cells were extracted and subjected to gene expression analysis.