Extensive variation in sperm morphology in a frog with no sperm competition

Background: Recent comparative studies of several taxa have found that within-species variation in sperm size decreases with increasing levels of sperm competition, suggesting that male-male gamete competition selects for an optimal sperm phenotype. Previous studies of intraspecific sperm length variation have all involved internal fertilizers where some other factors—e.g., sperm storage and sperm movement along the walls of the female’s reproductive tract—probably also influence and reduce sperm size variation. Thus external fertilizers, where those factors are absent, might be expected to exhibit even more variation when there is little or no sperm competition. To test that idea, we studied the sperm morphology of a North American chorus frog, the spring peeper (Pseudacris crucifer), a species in which males encounter little or no sperm competition. Results: As expected, sperm size was highly variable in the spring peeper, largely due to variation in flagellum length within and amo...