ZBP1 binds cytosolic DNA

ZBP1(DAI) binds to double-stranded DNA in vitro and in vivo (Wang ZC et al 2008; Takaoka A et al 2007). N-teminus of ZBP1 contains two Z-DNA (Zalpha and Zbeta) and one B-DNA binding domains (D3 region). D3 region mediates initial binding of ZBP1 to DNA with subsequent stabilization provided by the Zalpha and Zbeta domains. All tree DNA-binding domains are required for ZBP1 full activation (Wang ZC et al 2008). <p>ZBP1 was reported to form multimer upon DNA binding that might facilitate innate immune responces (Wang ZC et al 2008; Ha SC et al 2008).

ZBP1（DNA结合型AI）在体外和体内均能与双链DNA结合（王志成等，2008；高木昭孝等，2007）。ZBP1的N端包含两个Z型DNA结合域（Zalpha和Zbeta）以及一个B型DNA结合域（D3区域）。D3区域介导ZBP1与DNA的初步结合，而Zalpha和Zbeta域则提供随后的稳定化作用。ZBP1的三个DNA结合域均需具备，方能实现其完全激活（王志成等，2008）。研究表明，ZBP1与DNA结合后可形成多聚体，这或许有助于促进先天免疫反应（王志成等，2008；哈世成等，2008）。