Data_Sheet_1_Inkjet Printing-Based Immobilization Method for a Single-Step and Homogeneous Competitive Immunoassay in Microchannel Arrays.docx

In this study, we report an inkjet printing-based method for the immobilization of different reactive analytical reagents on a single microchannel for a single-step and homogeneous solution-based competitive immunoassay. The immunoassay microdevice is composed of a poly(dimethylsiloxane) microchannel that is patterned using inkjet printing by two types of reactive reagents as dissolvable spots, namely, antibody-immobilized graphene oxide and a fluorescently labeled antigen. Since nanoliter-sized droplets of the reagents could be accurately and position-selectively spotted on the microchannel, different reactive reagents were simultaneously immobilized onto the same microchannel, which was difficult to achieve in previously reported capillary-based single-step bioassay devices. In the present study, the positions of the reagent spots and amount of reagent matrix were investigated to demonstrate the stable and reproducible immobilization and a uniform dissolution. Finally, a preliminary application to a single-step immunoassay of C-reactive protein was demonstrated as a proof of concept.

本研究报告了一种基于喷墨打印技术的固定不同反应性分析试剂于单一微通道的方法，以实现单步和均相溶液式竞争性免疫分析。该免疫分析微器件由一个采用喷墨打印技术图案化的聚二甲基硅氧烷微通道构成，该通道上以可溶解点的方式固定两种反应性试剂，分别为抗体固定化的氧化石墨烯和荧光标记的抗原。由于纳米级试剂液滴能够精确且位置选择性地滴加到微通道上，因此能够在同一微通道上同时固定不同的反应性试剂，这在先前报道的基于毛细管的单步生物分析器件中难以实现。在本研究中，我们调查了试剂点的位置和试剂基质的量，以证明固定化的稳定性和可重复性，以及均匀的溶解性。最后，以C反应蛋白的单步免疫分析作为概念验证的初步应用。