首页/数据集/Assessing the orthogonality of various tRNA constructs in HEK293T cells.

Assessing the orthogonality of various tRNA constructs in HEK293T cells.

Figshare2015-12-02 更新2026-04-29 收录

数据链接：

https://figshare.com/articles/dataset/_Assessing_the_orthogonality_of_various_tRNA_constructs_in_HEK293T_cells_/745206 数据链接链接失效反馈

官方服务：

资源简介：

72 hours after transfection, FACS was used to measure the population of fluorescent cells.

应用场景：

创建时间：

2015-12-02

基因治疗

tRNA工程

PTCs cause a multitude of human diseases and there are no established therapeutic options for their therapeutic management. Herein, we report the high-throughput cloning and identification, character

NIAID Data Ecosystem50

Engineered tRNAs suppress nonsense mutations in cells and in vivo

基因治疗

tRNA工程

Comparison of the overall tRNA expression level and detection of the suppressor tRNA Mice were transfected with suppressor tRNA and sacrificed after 6 or 72 h. RNA from mice liver were analyzed to det

NIAID Data Ecosystem20

PROTein Evolution Using Selection (PROTEUS) [Illumina]

蛋白质定向进化

哺乳动物细胞合成生物学

Directed evolution (DE) is a process of mutation and iterative artificial selection to breed biomolecules with new or improved activity. DE platforms are primarily prokaryotic or yeast-based, and stab

NIAID Data Ecosystem10

Engineered tRNAs suppress nonsense mutations in cells and in vivo. Engineered tRNAs suppress nonsense mutations in cells and in vivo

基因治疗

tRNA工程

Comparison of the overall tRNA expression level and detection of the suppressor tRNA Overall design: Cells transfected with suppressor tRNA and time samples analyzed to determine the half-life time of

NIAID Data Ecosystem40

Engineered mischarged transfer RNAs for correcting pathogenic missense mutations [MSR-seq]. Engineered mischarged transfer RNAs for correcting pathogenic missense mutations [MSR-seq]

tRNA工程

突变校正

Missense mutations account for nearly 50% of pathogenic mutations in human genetic diseases, most lack effective treatments. Gene therapies, CRISPR-based gene editing, and RNA therapies including tran

NIAID Data Ecosystem10

Assessing the orthogonality of various tRNA constructs in HEK293T cells.

资源简介：

相关数据集