Quantification of antennal lobe defects in sema1a knockdown animals.

The above set of data represents a compiled summary of results obtained for control-fed vs. sema1a knockdown (KD) animals from a total of eight replicate experiments performed in both larvae (left) and pupae (right). The total number of animals assessed (n) and numbers/percentages of animals displaying wildtype morphology vs. antennal lobe (AL) defects (olfactory sensory neuron targeting defects, lack of neuropil and glomeruli formation) are indicated. Knockdown was immunohistochemically confirmed through anti-Sema1a antibody staining in a subset of animals (15 larvae and 10 pupae) displaying the most severe defects (denoted with *). All KD animals assessed in this manner were found to have reduced levels of Sema1a, while Sema1a levels in control-fed animals were normal (examples are shown in Figures 2 and 7).