ChIP-Seq based CTCF binding profiling of naïve B-cells from CTCFfl/fl; CD19-Creki/+ or CTCFfl/+; CD19-Creki/+ mice.

The goal of this study was to compare the CTCF binding expression profile, using ChIP-seq technology, of naïve B-cells from CTCFfl/fl; CD19-Creki/+ or CTCFfl/+; CD19-Creki/+ mice. Naïve B cells, isolated from the spleen of CTCFfl/fl; CD19-Creki/+ or CTCFfl/+; CD19-Creki/+ mice, were used to extract genomic DNA with the aim of studying CTCF binding site occupancy using ChIP-Seq. C* samples have genotype CTCF fl / +; CD19-Cre ki/+; they are heterozygous for the Ctcf-floxed allele. F* samples have genotype CTCF fl / fl; CD19-Cre ki/+; they are homozygous for the Ctcf-floxed allele. CTCF Antibody (Diagenode, C15410210-10, lot A2359-00234P)