The impact of MET2 gene deletionon on the morphological transformation of C. albicans SC5314 cells

The datasets present results on the impact of MET2 gene deletion, encoding homoserine acetyltransferase, on the morphological transformation of C. albicans SC5314 cells.
Overnight cultures of C. albicans were washed twice with sterile phosphate-buffered saline (PBS). The cell suspensions were diluted to 2 × 10⁵ cells mL⁻¹ in medium. A 100 μL aliquot of the cell suspension was added to 100 μL of the appropriate medium in a 96-well microplate. The microplates were incubated at 37 °C for 3 h, and images were obtained using a TECAN Spark 10M microplate reader. Additionally, a 10² cell dilution was spread onto solid medium. The plates were incubated at 37 °C for 6 days.
Media used:
SLAD medium (Synthetic Low Ammonium Dextrose) consists of 1.7%  (w/v) yeast nitrogen base without amino acids and ammonium sulfate, 2% (w/v) glucose, and 50 µM ammonium sulfate as the sole nitrogen source, 2% (w/v) agar.
Spider medium consists of 1% (w/v) nutrient broth, 1% (w/v) mannitol, and 0.2% (w/v) dibasic potassium phosphate (K₂HPO₄), adjusted to pH 7.2, with 2% agar added for solid medium.

GlcNAc medium consists of 0.5% (w/v) N-acetylglucosamine (GlcNAc), 0.5% (w/v) peptone, and 0.3% (w/v) potassium dihydrogen phosphate (KH₂PO₄).
YEPG medium consists of 1% (w/v) yeast extract, 2% (w/v) peptone, and 2% (w/v) glucose as the carbon source, 2.5% (w/v) agar and with 10% FBS (Fetal Bovine Serum) added.
RPMI 1640 medium consists of a balanced mixture of amino acids, vitamins, inorganic salts, and 2% (v/v) glucose, buffered with 0.165 M MOPS (pH 7.0) with 10% FBS (Fetal Bovine Serum) added.

The studies were financially supported from the project OPUS 20 financed by the National Science Centre (No. UMO-2020/39/B/NZ7/01519).