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mRNA stability experiments with control or CNOT1-targeting siRNA treatment in HEK293 cells

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NIAID Data Ecosystem2026-03-13 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE158619
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资源简介:
Determination of mRNA half-life in HEK293 cells with and without the depletion of CNOT1. HEK293 cells were treated with control or CNOT1-targeting siRNA for 48hrs. Transcription was then inhibited with 1uM triptolide and RNA samples taken at multiple time points (0, 0.5, 1, 2, 4, 8 & 16 hours after transcriptional inhibition). Poly(A) selected RNA was then subject to RNA-seq. This was conducted for three biological replicates.
创建时间:
2021-12-12
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