Bioelectrochemical detection of COVID protein (RBD) on boron doped diamond - optimization of functionalziation parameters

The following dataset is a comprehensive overview of preparation of biofunctionalizaed BDD (boron-doped diamond) electrodes and their application in electrochemical sensing of COVID protein RBD. The specific receptor against RBD (Receptor Binding Domain) protein ACE-2 (Angiotensin Convertase Enzyme) is attached to the surface of BDD via a series of modification steps (diazonium electrografting, EDC/NHS linkers, and ACE-2 proteins). Functionalization parameters (linker concentrations, incubation times) are optimized towards optimal sensing performance. Influence of BSA adsorption after functionalizations on RBD detection is investigated. Stability and selectivity of the functionalziation is verified. Alternative route of functionalziation via his-tag was tested with negative results.