High fat diet enriched in eicosapentaenoic and docosahexaenoic acids delays the development of fatty liver in mice

To investigate the effects of quality of fat in a high fat diet (HFD) over time on hepatic lipid storage and transcriptome in mice. In this dataset, we include the expression data obtained from dissected mouse liver after being fed with Control, HFD-EPA/DHA and HFD-corn oil diet for 8 and 12 weeks. In total, 24 samples were analyzed. Based on hierarchical clustering and scatter plots, one microarray from the HFD-corn oil group at 8 weeks was removed from subsequent analyses. The Piano package was used for Gene Set Enrichment Analysis (GSEA) to identify the total number of genes regulated and the direction of regulation. Gene Ontology (GO) terms were annotated to each probe-set after performing GSEA. The reporter algorithm was used to analyse the functional enrichment level of individual GO term. Heatmaps were generated using the log10p-values to visualize enriched GO biological processes (BPs) terms.本研究旨在探究高脂饮食（High Fat Diet, HFD）中脂肪品质随时间变化对小鼠肝脏脂质储存与转录组的影响。本数据集包含分别以对照饮食、HFD-EPA/DHA饮食及HFD-玉米油饮食喂养小鼠8周和12周后，解剖获取的小鼠肝脏组织表达谱数据。本次分析共纳入24个样本；基于层级聚类与散点图结果，我们移除了8周龄HFD-玉米油组的1个芯片样本，后续分析不再包含该样本。本研究使用Piano工具包开展基因集富集分析（Gene Set Enrichment Analysis, GSEA），以鉴定受调控基因的总数及其调控方向。完成GSEA后，我们为每个探针集注释了基因本体（Gene Ontology, GO）条目。采用报告者算法对单个GO条目的功能富集水平进行分析。我们以log₁₀转换后的P值为数据源绘制热图，以可视化富集的GO生物过程（Biological Processes, BPs）条目。