Refrigerated storage of dusky grouper Epinephelus marginatus sperm

ABSTRACT This study aimed to evaluate different extenders in refrigerated storage of dusky grouper sperm. Initially, motility rate, motility time, and sperm density were analyzed to characterize the fresh sperm quality. Different diluents were used in the refrigerated storage sperm at 4ºC in normal atmosphere and modified atmosphere (100% oxygen). The fresh sperm has a spermatozoa density of 3.1±0.2 x 109 spermatozoa mL-1, motility rate 90% and motility time of 3,060 seconds. In the experiment of refrigerated storage, the motility rate and the motility time were maintained appropriate for 144 hours for the extender A (70%; 3,100 seconds) in normal atmosphere. In the modified atmosphere, the sperm quality decreased drastically during the first 24 hours, independently of the diluent used, not propitiating advantages. The refrigerated sperm of dusky grouper is a viable alternative for the short-term storage, being possible, to maintain for up to 144 hours an appropriate sperm viability.摘要 本研究旨在评估不同精子稀释剂（extender）在暗点石斑鱼（dusky grouper）精子冷藏保存中的应用效果。首先通过分析精子活力率、运动时长及精子密度，对新鲜精子的品质进行表征。随后分别在常压及改性气氛（100%氧气）条件下，以4℃冷藏储存精子，并使用不同稀释剂进行处理。新鲜精子的精子密度为3.1±0.2×10^9 精子/mL，活力率为90%，运动时长为3060秒。在冷藏储存实验中，常压条件下使用稀释剂A时，精子活力率与运动时长可在144小时内维持适宜水平（70%；3100秒）。而在改性气氛条件下，无论使用何种稀释剂，精子品质均在最初24小时内急剧下降，未体现出应用优势。暗点石斑鱼的冷藏精子可作为短期储存的可行方案，能够将适宜的精子活力维持长达144小时。