Expression of TWIST1, mesenchymal (CDH2, VIM, SNAI1, ZEB1) and epithelial (CDH1, JUP) markers in lung adenocarcinoma cell lines.

Assessment of TWIST1, CDH2 (N-Cadherin), VIM (Vimentin), SNAI1 (SNAIL1), ZEB1, CDH1 (E-Cadherin) and JUP (plakoglobin) transcriptional expression by quantitative RT-PCR. The relative expression levels between samples were calculated using the comparative delta Ct (threshold cycle number). In each run, in addition to study samples, normal human lung RNA was used as calibrator. Each value represents the mean ±SD of three independent experiments each one run in triplicate.