DataSheet1_Cancer organoid applications to investigate chemotherapy resistance.pdf

In clinical practice, a large proportion of cancer patients receive chemotherapy, yet tumors persist or acquire resistance; removing this obstacle could help to lower the number of cancer-related fatalities. All areas of cancer research are increasingly using organoid technology, a culture technique that simulates the in vivo environment in vitro, especially in the quickly developing fields of anticancer drug resistance, drug-tolerant persisters, and drug screening. This review provides an overview of organoid technology, the use of organoids in the field of anticancer drug resistance research, their relevance to clinical information and clinical trials, and approaches to automation and high throughput.

在临床实践中，大量癌症患者接受化疗，然而肿瘤仍持续存在或产生耐药性；克服这一障碍有望降低癌症相关死亡人数。癌症研究的各个领域正日益采用类器官技术，这是一种模拟体内环境的体外培养技术，尤其在抗癌药物耐药性、药物耐受性持久存在和药物筛选等迅速发展的领域。本文综述了类器官技术，类器官在抗癌药物耐药性研究领域中的应用，其与临床信息和临床试验的相关性，以及自动化和高通量方法的探索。