Yarrowia keelungensis strain CBS 11062 genome sequence. Yarrowia keelungensis strain CBS 11062 genome sequence

The genome of Yarrowia keelungensis strain CBS 11062 has been sequenced using Illumina Solexa technology with a shot-gun library and a mate pair library of 8 kb in average. The raw reads were trimmed with Trimmomatic and cutadapt. The assembly was done using SOAPdenovo2 with a kmer of 71, as estimated with kmergenie. Two successive runs of GapCloser were used to close gaps. The current draft genome sequence is composed of 40 scaffolds larger than 5 kb (39 nuclear and 1 mitochondrial ones), for a total size of the nuclear DNA of 21,639,051 bp with an N50 of 1,074,350 bp (6 scaffolds) and a G+C content of 48.2%. Annotation of protein-coding genes was performed using the Amadea Annotation transfer tool (Isoft, France), with Yarrowia lipolytica genome as reference. The structural annotation of these genes was manually curated using RNA-Seq data and other genetic elements (tRNA and transposable elements) were expertly added.