Expression profile (nanoCAGE-seq), chromatin accessibility (ATAC-seq) profile, and histone modification profile (ChIP-seq for H3K4me3, H3K9me3, and H3K27me3) during the course of mouse gonocyte development

How de novo DNA methylation over heterochromatin is introduced in mouse gonocyte remains obscure. We performed nanoCAGE-seq, ChIP-seq for three major histone marks (H3K4me3, H3K9me3, H3K27me3), ATAC-seq, Hi-C during the course of mouse gonocyte development (E13.5, E17.5, E19.5, P2) The FACS-sorted Mvh-Venus cells from E13.5, E17.5, E19.5, or P2, are used as starting material. Using each sample, ATAC-seq, ChIP-seq, and Hi-C library preparation was performed in duplicate. For nanoCAGE-seq analysis, the experiments were done in triplicate. All DNA libraries were sequenced by Illumina MiSeq or HiSeq.