Sugar-responsive Mondo/Mlx-target genes.

Previous study has reported sugar-dependent transcriptomes in wild-type and mutants of max-like protein X (mlx, also known as bigmax), the obligated partner of Mondo [6]. To identify Mondo/Mlx-target genes, RNA-seq datasets reported in (GES70980) [6] were analyzed using the FlyBase reference genome (Dmel Release 6.19). First, we selected genes whose expression levels were significantly changed between control and mlx mutant larvae under high sugar conditions. Of those, genes whose expression levels were significantly different between control and mlx mutants under low sugar conditions were removed. control_HSD = average expression levels of triplicated experiments of HSD-fed control larvae (FPKM), mlx1_HSD = average expression levels of triplicated experiments of HSD-fed mlx mutant larvae (FPKM), logFC = log2 fold-change, test_stat = test statistics, p_value = uncorrected p-value of the test statistics, q_value = adjusted p-value of the test statistics with Benjamin–Hochberg correction. (XLSX)