Establish the male meiocyte-specific epigenomic landscape

Histone modification maps of H3K4me3 and H3K27me3 of meiocytes were obtained by microlibrary and high-throughput sequencing by extracting meiocytes from rice varieties Azucena and IR64. And the DNA methylation results of meiocytes and flag-leaf of Azucena and IR64 were obtained. By collating this data, the male meiocyte-specific epigenomic landscape is constructed Overall design: By micromanipulation technology, the meiocytes of Azucena and IR64 were obtained, respectively. Libraries were constructed by CUT&Tag technology to obtain histone modification data. DNA methylation libraries were constructed by scBS-seq technology. In addition, flag-leaf's DNA methylated library is constructed by directly extracting whole genome DNA