Single-cell multi-omics decodes regulatory programs during development of mouse secondary palate

The abnormal perturbation in gene regulation during palatogenesis may lead to cleft palate. Here, we performed single-cell multiome sequencing (snRNA-seq and snATAC-seq simuteneously from same cells) from mouse secondary palate across 4 developmental stages. Palatal shelves were isolated from time-mated C57BL/6J mice (Jackson Laboratory) at embryonic day (E) 12.5, E13.5, E14.0, and E14.5 and subjected to single-cell multiome sequencing. All mice were maintained in the animal facility of UTHealth. The protocol was approved by the Animal Welfare Committee (AWC) and the Institutional Animal Care and Use Committee (IACUC) of UT Health (AWC 19-0079).腭发育过程中基因调控的异常扰动可导致腭裂。本研究针对4个发育阶段的小鼠次级腭组织开展单细胞核多组学测序，即对同一细胞同步开展单细胞核RNA测序（snRNA-seq）与单细胞核转座酶可及性测序（snATAC-seq）。我们从定时交配的C57BL/6J小鼠（杰克逊实验室）的胚胎第12.5天（E12.5）、E13.5、E14.0及E14.5阶段分离腭突，并对其进行单细胞核多组学测序。所有小鼠均饲养于UTHealth的动物实验设施中，本实验方案已通过UTHealth动物福利委员会（Animal Welfare Committee, AWC）与机构动物护理与使用委员会（Institutional Animal Care and Use Committee, IACUC）的审批，审批编号为AWC 19-0079。