Identification of bacterial genera in lake water using Platinum Direct PCR Universal Master Mix

Genomic DNA extraction step can be time consuming and carries a risk of losing metagenomic materials required for bacterial identification. Invitrogen Platinum Direct PCR Universal Master Mix eliminates gDNA extraction steps by enabling DNA amplification directly from biological samples. We present a method to study bacterial genera in lake water samples without the need to purify gDNA samples, thereby reducing the risk of sample loss and workflow time, using Platinum Direct PCR Universal Master Mix. We also provide an experimental design for direct amplification with 16S primers, followed by NGS of PCR amplicons, and sequencing data analysis