Effect of cell passage number on gene expression in NR8383 macrophages

Continuous cell lines are important research tools in biology and medicine. However, literature demonstrates that over-subculturing changes cell lines properties over time. In addition, cultured cells adapt to stress and confusion by evolution - both genotypic and phenotypic. The aim of this study is to identify differentially expressed genes and key biological pathways at high-passage cells compared to lower-passage cells. To do that, we performed microarray analyses on NR8383 macrophages at 20 and 200 passages numbers. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 686098 Changes in gene expression in NR8383 cells over times were measured. Cells at low (passage 20) and high (passage 200) passage numbers are used. For each condition, four biological replicates were performed: P 20-1; P 20-2; P 20-3; P 20-4 and P 200-1; P 200-2; P 200-3, P 200-4.