Astrocyte-derived extracellular vesicles enhance the electrophysiological function and cell survival of human cortical neurons in vitro

The immunostained image analysis of neurons created, NTA analysis of obtained EVs, image-based demonstration of EV-uptake by neurons, FACS analysis to quantify apoptotic behavior of cultures of interest, beta-galactosidase result to explain cell senescence status in each group, patch clamp experiment result to demonstrate the electrophusiological effect of astroglial EV treatment.

构建神经元免疫染色图像分析，对获得的外泌体进行 NTA 分析，展示神经元对外泌体摄取的图像化过程，利用 FACS 技术对目标细胞培养的凋亡行为进行量化分析，通过 β-半乳糖苷酶检测结果阐释各组细胞衰老状态，以及通过电生理实验结果展示星形胶质细胞外泌体治疗对电生理效应的影响。