ATHB17Δ113 can bind both Class II and Class I DNA targets and ATHB17Δ113 containing V182A-Q185A-N186A mutation cannot bind Class II DNA target in in vitro assay (measured by Surface Plasmon Resonance (SPR).
收藏Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_ATHB17_113_can_bind_both_Class_II_and_Class_I_DNA_targets_and_ATHB17_113_containing_V182A_Q185A_N186A_mutation_cannot_bind_Class_II_DNA_target_in_in_vitro_assay_measured_by_Surface_Plasmon_Resonance_SPR_/1000569
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Binding affinities and Kinetic constants of ATHB17Δ113 interacting with Class I and Class II type DNA, measured by Biacore 2000, globally fitted. SPR measurements with Biacore 2000 were at 25°C in HBS-EP, 100 ug/ml BSA (10 mM HEPES pH 7.4, 150 mM NaCl, 3 mM EDTA, 0.005% Tween-20,100 ug/ml BSA). Equilibrium dissociation constant KD = koff/kon.
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2015-12-02



