DNA methylation and gene expression profiles of hypothalamus in ewe

we established the ovariectomized, estradiol-implanted (OVX+E2) ewes which were maintained in artificially controlled lighting room. Secretion patterns of serum reproductive hormone were detected. Dynamic DNA methylation and gene expression profiles of hypothalamus were measured at different time points using OVX+E2 sheep under artificial short-day (SP) or long-day (LP). The landscapes of the DNA methylome under SP as compared to LP were investigated