Patient-derived lymphomoids preserve the tumor architecture and allow testing response to therapies in lymphoma [Spatial Transcriptomics]

We designed an ex vivo system to culture tissue explants from lymphoma tissue. To characterize the spatial and molecular structure of human lymphomoids and their tissue of origin, we performed spatial transcriptomics analyses on original tissues and lymphomoids obtained from 2 patients using the 10x Genomics Visium technology. Overall design: Two lymph node biopsies were used for this analysis. One biopsy was taken from a patient with DLBCL (patient 08 in this study) and a second biopsy contained reactive lymphoid tissue ( (patient 03 in this study). In addition, for patient 08, we analysed tissue explants grown for 3 days untreated or treated with 2 mM ibrutinib or 10 mM idelalisib.