Simultaneous and sensitive quantification of protein and low molecular weight persulfides, polysulfides, and H2S in biological samples

This work describes the development and validation of a high-sensitivity analytical method (LC-MS/MS IAM-TPP) for the simultaneous quantification of protein persulfides, low molecular weight persulfides/polysulfides and hydrogen sulfide (H₂S) in complex biological matrices. The method was applied to biological samples to characterise H2S and endogenous persulfide/polysulfide formation, including analysis or physiologically relevant ischemia reperfusion models. The raw dataset is intended to enable independent re-analysis, method reuse, and further translational development in redox biology and drug discovery.

Methods
The data derive from experiments described in the manuscript: using the IAM-TPP technique, persulfide and polysulfide species were trapped, derivatised, separated by liquid chromatography, and quantified by tandem mass spectrometry. Sample preparation, calibration curves, and validation experiments (linearity, limit-of-detection, reproducibility) are detailed in the publication.

Limitations: The dataset reflects specific sample types, instruments, and experimental conditions; normalisation and instrument-specific settings may differ in other laboratories. Raw data are provided to enable inspection of processing pipelines.