Medicago_superpangenome

We performed de novo genome assembly and annotation for the autotetraploid alfalfa cultivar 'Zhongmu No.4', resulting in a 3.13 Gb genome assembled into 32 chromosomes with a contig N50 of 5.45 Mb, named ZM4_V2.0. Furthermore, we constructed a super-pangenome using 13 genomes from seven Medicago taxa. This included a de novo assembly and annotation of M. sativa subsp. caerula (Mca_long) and the re-annotation of 11 previously published genomes. The M. truncatula A17 (v5.0) genome and annotation served as the reference for this analysis.