Effect of depletion of SIRT6 in gene expression in Nucleus pulposus cells

SIRT6 is a well known histone deactylase enzyme. Here, we report the effect of its depletion in nucleus pulposus cells from rattus norvegicus. RNA seq was performed on control and sirt6 depelted cells via lentiviral transfection method. Downstream analysis includes upregulated and downregulated signalling molecules with respect to SIRT6 depletion. SIRT6 depletion was achieved via lentiviral transfection method via ShRNA on Nucleus pulposus cells derived from four(n=4) rats (rattus norvegicus) and submitted to RNA sequencing. Thereafter, comparative gene expression profiling was performed on SIRT6 depeleted cells(SIRT6KD/ShSirt6) vs their respective controls (SIRT6Ctrl/ShCtrl).