Calculation of virus replication cycles during selection.

1AUC; area under curve for cumulative increase in capsid p24 antigen shown in Fig. 2A for the indicated number of weeks (first to last).2Calculation of replication cycles assume 1 cycle/day for HIV-1 CC1/85 in control cultures [66], and is corrected for weeks of replication (i.e., allowing for pauses) and diminished p24 levels (% control) for cultures under 5P12-RANTES (5P12) selection.3Expected mutations are calculated based on the rate found by Abram et al.[54] of 1.4×10−5 mutations/bp/cycle and the 2553 bp target envelope gene.4Observed mutations are the mean number of nucleotide mutations observed in all envelope molecular clones with confirmed entry via CXCR4.