Streptomycin-induced Changes to the Intestinal Microbiome Alter the Pulmonary T Cell profile and Airway Hyperresponsiveness in a Cystic Fibrosis Mouse Model

Here we describe the small intestinal microbiome in a cystic fibrosis mouse model treated or untreated with streptomycin. We used 16S rRNA gene sequencing of 12 week old BALB/c Cftrtm1UNC mice and aged and sex matched wild-type littermates, to characterize the community membership. For the streptomycin treated groups, experimental mice and their direct progenitors were maintained on streptomycin in their drinking water. We detected microbial dysbiosis between untreated cystic fibrosis and wild-type mice, which was altered with streptomycin treatment. This resulted in a altered pulmonary T cell profile and reduce airway hyperresponsiveness in cystic fibrosis mice