LPS-stimulated endothelial cells using SILAC

Severe sepsis and septic shock, which occur with high incidence and are prevalent in the emergency department setting, are among the most common causes of death in hospitalized patients worldwide, and there has been a continuous increase in the incidence of sepsis of approximately 5–10% per year. Endothelial cells constitute an important metabolic organ in the physiological homeostasis of blood vessels and play a pivotal role in orchestrating the inflammatory response triggered by sepsis or endotoxemia. The secretome is defined as the subset of the proteome that contains all the proteins actively exported by a cell following a particular event.