A Patient-Derived Organ-on-Chip Platform for Modeling the Tumor Microenvironment and Drug Responses in Pancreatic Cancer

RNA sequencing data was generated from Transwell based co-culture experiments. Two independent co-culture experiments were performed. In the first, primary fibroblasts were cultured in the lower chamber and co-cultured with patient-derived pancreatic cancer organoids (PDOs) or Matrigel-only controls in the upper insert, allowing tumor - stroma communication. RNA was extracted from stellate cells after co-culture.In the second experiment, tumor models consisting of PDOs, pancreatic stellate cells, and monocytes were placed in the upper insert, while activated human T cells were cultured in the lower chamber. T cells cultured with Matrigel-only inserts served as controls. RNA was extracted from T cells to assess transcriptional responses to tumor-derived signals.All samples were processed for bulk RNA sequencing. Fastq files are deposited here.