File S1 - Three Mitogen-Activated Protein Kinases Required for Cell Wall Integrity Contribute Greatly to Biocontrol Potential of a Fungal Entomopathogen

File includes Figures S1–S3 and Tables S1–S2. Figure S1. Conserved domains located in the Bck1 (A), Mkk1 (B) and Slt2 (C) sequences of B. bassiana (Bb2860). Figure S2. Phylogenetic tree constructed for the Bck1 and Mkk1 and Slt2 homologues of B. bassiana and selected fungi with MEGA5. The bootstrap values of 1000 replications are given at nodes. Figure S3. Generation and identification of B. bassiana bck1, mkk1 and slt2 mutants. (A–C) Diagrams for the disruptions of bck1, mkk1 and slt2 respectively. (D–F) The mutants of bck1, mkk1 and slt2 identified via PCR (Lanes 1–6) and Southern blotting (Lanes 7–9) analyses of genomic DNAs with paired primers and amplified probes (Table S1) respectively. Lanes 1, 4 and 7: WT. Lanes 2, 5 and 8: disruption mutant. Lanes 3, 6 and 9: complemented mutant. Table S1. Paired primers designed for the manipulation of B. bassiana bck1, mkk1 and slt2. Table S2. Genes and paired primers used in qRT-PCR for assessments of their transcripts in B. bassiana cultures under different conditions.
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