RNA-sequencing of Col17a1 tumor isolated from FVB mice. Mus musculus

Expression of COL17A1 mRNA encoding collagen XVII is associated with worse or better patient survival depending on cancer types. However, little is known of the underlying mechanisms of a context-dependent effects of collagen XVII on cancer progression. To test this, a murine pancreatic cancer cell line AK4.4 was transduced with Col17a1-expressing vector (AK4.4-Col17a1) or corresponding control vector (AK4.4-vector). Mouse tumorigenicity assay showed that the overexpression of Col17a1 suppressed the growth of AK4.4-Col17a1 tumor relative to AK4.4-vector tumor in recipient FVB mice. To explore the mechanisms of how Col17a1 suppresses the tumorigenicity of AK4.4, we performed RNA-sequencing analysis of AK4.4-Col17a1 and AK4.4-vector tumors isolated from FVB mice.