James R. LaFountain, Rudolf Oldenbourg (2011) CIL:12026, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset

Movie of meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis that have been experimentally flattened.
Time-lapse polarization microscopy using a Nikon
Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 30/60/90 sec for 2 hr 31 min using a QImaging Retigo EXi CCD camera. Raw images were processed using a 5-frame algorithm (Shribak and Oldenbourg, 2003). A movie of the time series is included in this grouped set.

本数据集包含对蜻蜓科蜻蜓属Nephrotoma suturalis初级精母细胞减数分裂I期的电影记录。采用Nikon Microphot SA显微镜，配备液晶偏振光显微镜系统（LC-PolScope，CRi，Woburn，马萨诸塞州），60倍/1.4 PlanApo油浸物镜，1.4 NA油浸聚光镜，以及2.0倍放大镜头进行时间间隔偏振显微镜观察。利用QImaging Retigo EXi CCD相机，每30秒、60秒或90秒捕获一次图像，持续2小时31分钟。原始图像经过5帧算法处理（Shribak and Oldenbourg, 2003）。该组数据集包含时间序列的电影。