Bos taurus Transcriptome or Gene expression

In this study, we prepared high-purity bovine interferon gamma dimer protein using prokaryotic expression system and affinity chromatography. We subsequently investigated the effect of BoIFN gamma on BoHV-1 infection in Madin-Darby bovine kidney (MDBK) cells. The results showed that BoIFN gamma pretreament not only decreased the production of BoHV-1, but also reduced the cytopathic effect of the virus. Differential gene expression profiles of BoHV-1 infected MDBK cells were then analysed through high-throughput RNA sequencing to analyze its molecular mechanism.