Next Generation Sequencing of wild-type and ORP4L knock-in murine T-cells Transcriptomes

Purpose: The goals of this study is to determine whether specific gene sets are dysregulated in the murine ORP4L knock-in T-cells, as compared to wild-type T-cells. Methods: T-cell mRNA profiles of wild-type and ORP4L knock-in mice were generated by deep sequencing, using Illumina NovaSeq 6000 sequencer for 318 cycles.Reads that passed the Illumina quality filters were kept for the subsequent analyses. Adapters were trimmed from the reads, and reads shorter than 17 nt were discarded. The reads were mapped to the Mouse mRNA reference database using FANSe3 algorithm on Chi-Cloud NGS Analysis Plantform. Results: We use edgeR to analysis ORP4 kncok-in vs. wild-type，with a |log2 (FoldChange)| > 1 and p value <0.01. Hierarchical clustering of differentially expressed genes uncovered a total of 970 differentially expressed genes (DEGs) , of which 539 were down-regulated and 431 up-regulatedseveral. Conclusions: Our study represents the first detailed analysis of transcriptomes that promote T-cell malignant transformation driven by oncogene ORP4L in mice. Examination of transcriptomes in T-cells from wild-type and ORP4L knock-in mice.