RNA-seq of murine breast cancer cell line AT3 infected with F. nucleatum

Fusobacterium nucleatum (F. nucleatum) is implicated to exacerbate colorectal cancer. However, there is also evidence that the bacterium accumulates on other cancer types such as breast cancer. In order to better understand the transcriptional response of breast cancer cells to infection with F. nucleatum, we performed RNA-seq of the murine breast cancer cell AT3 with F. nucleatum. Cells were seeded 2 days prior to infection and from this point on kept in an oxygen-controlled incubator (1% oxygen). The DMEM media was exchanged after 24h not containing otherwise used antibiotic mix. On the second day, AT3 cells were infected with F. nucleatum (m.o.i. of 10). Three biological replicates of infected and mock control samples were taken 24h after infection.