Amplicons for NGS were prepared following cloning of PCR fragments into mammalian expression vectors as outlined in Fig 8A. The average number of full-length reads was determined based on the percenta

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Sequences of primer pairs for the amplification of the five PAV elements; PAV1, PAV2, PAV3, PAV4 and PAV5 (Fig 1).

PAV元件扩增

PCR引物设计

Sequences of primer pairs for the amplification of the five PAV elements; PAV1, PAV2, PAV3, PAV4 and PAV5 (Fig 1).

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PCR primer pairs used for DNA methylation analysis.

DNA甲基化分析

PCR引物设计

aall forward primers (top) included the following common sequence at the 5′ end: AGGAAGAGAG; all reverse primers (bottom) the sequence CAGTAATACGACTCACTATAGGGAGAAGGCT.

Figshare2015-12-02 更新30

Primers used for PCR and qRT-PCR amplification.

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