Transcriptome analysis of ABCB7 overexpressed U87MG glioblastoma cells

This study investigated the biological function of ABCB7 in U87MG glioblastoma cell line. Many ABC transporters are known contributions to drug resistance and various biological process. However, mitochondrial ABC transporters aren't known mechanism to control biological process. We identified the potential roles of ATP-binding cassette (ABC) transporter subfamily member 7 (ABCB7), one of the mitochondrial ABC transporters. ABCB7 activates signaling pathway of hypoxia-inducible factor 1 alpha (HIF1a) by hypoxia independent regulation. This signaling pathway regulates the death inhibitory genes and survival related genes. Also, ABCB7 downregulates cell cycle related genes. Our results provide that ABCB7 is essential for the survival of malignant brain tumors. Overall design: A Glioblastoma cell line, U87MG, was transfected with pCDH-CMV-MCS-EF1-Puro (U87MG-Con) or pCDH-CMV-ABCB7-EF1-Puro (U87MG-ABCB7). Plasmids for ABCB7 expression (NM_004299.6) were purchased from Korea human gene bank (hMU008618). The 3 biological replicate of RNA extracts in these cells was used for transcriptome analysis.