Effects of endocrine-active compounds on protein abundance of Drosha, DGCR8 and Dicer in the uterus of sow-fed and formula-fed piglets - raw data

This dataset derives from a study examining whether neonatal exposure to endocrine-active compounds that interfere with androgenic and estrogenic signaling alters the protein abundance of key components of the microRNA biogenesis machinery (Drosha, Dicer, and DGCR8). A central objective was to determine whether these effects are modulated by early postnatal nutrition, comparing piglets nourished with maternal milk to those fed a milk replacer.
To address this objective, we employed an ex vivo model to assess the solely effects of the antiandrogen 2-hydroxyflutamide, the environmental estrogen 4-tert-octylphenol, and the organochlorine insecticide methoxychlor metabolite 2,2-bis-(p-hydroxyphenyl)-1,1,1-trichloroethane (HPTE), which exhibits mixed steroidal properties (estrogenic, antiestrogenic and/or antiandrogenic), on the protein abundance of Drosha, DGCR8 and Dicer in uterine explants from 10-day-old sow-fed or formula-fed piglets. The dataset comprises semi-quantitative protein expression data for Drosha, DGCR8, and Dicer obtained by Western blot analysis.