Jennifer Bestman, Holly Cline (2011) CIL:37074, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #7 (hour 8) of a developing neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.
The cell was imaged at a 2 hour interval over 8 hours and shows the development and morphological plasticity of the dendritic arbor and axon over time. This is the second image of this neuron at this timepoint

The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

此为来自无色斑马鱼神经胚的视束发育神经元的第7张图像（8小时时），处于第47期。该细胞在2小时的时间间隔内连续8小时进行成像，展现了树突树和轴突随时间推移的发育与形态可塑性。这是该神经元在此时间点的第二张图像。
该细胞在图像采集前约24小时通过活体内电穿孔转染编码绿色荧光蛋白（eGFP）的质粒DNA。完整的活体蝌蚪经过麻醉，放置于载玻片下，通过特制的双光子显微镜直接穿透其透明皮肤进行成像。