Vitamin D receptor ChIP-Seq in primary prostate epithelial cells

We investigated the genomic activity of vitamin D in primary prostate epithelial cells (PrE) through vitamin D receptor (VDR)-ChIP-sequencing. PrE cells treated with 50 nM 1,25D showed nuclear localization the VDR within 1 hr, persisting until 4 hr, and a 2 hr treatment was selected for ChIP-seq. Rigorous testing of the ChIP conditions and VDR antibody were performed. Nearly 5,000 VDR binding sites were identified. One primary prostate epithelial cell line treated with ethanol (control) or 50 nM 1,25-VitaminD3 for 2 hr