Gene expression analysis in liver and BAT of wildtype and HSL null mice.

Data were generated using Real-time quantitative PCR on female mice after HFD followed by an overnight fast (12 h). Values given are arbitrary means ± SEM, n = 5. Relative mRNA quantities were normalized by geometric averaging of three internal control genes (RPS29, HPRT1 and GAPDH) for the liver and two internal control genes (RPS29 and TBP) for BAT. Differences between the two genotypes were analyzed using a non-parametric Mann-Whitney U-test. Data were considered significant if p*p**p